FC309 is a sugar beet (Beta vulgaris spp. vulgaris) pre-breeding line released from the USDA-ARS Fort Collins Sugar Beet Genetics Lab. FC309 is a derivative of an unreleased line from the USDA-ARS Salinas, California pre-breeding program (7927-4-309) selected from C927-4 (PI 628756). FC309 germplasm is the result of strong selection pressure for resistance to Fusarium Yellows (caused by Fusarium oxysporum) followed by single seed decent. The FC309 seed lot is diploid, self-fertile, segregating for hypocotyl color and multigerm seed, has uniform strong resistance to Fusarium Yellows, and susceptibility to Rhizoctonia Crown and Root Rot and Sugar Beet Cyst Nematode.