This dataset contains physiological measurements from a controlled laboratory experiment on juvenile Pseudotsuga menziesii (Douglas fir) conducted between June and August 2023 at the experimental greenhouse facility of the Karlsruhe Institute of Technology (KIT), Campus Alpin, Garmisch-Partenkirchen, southern Germany. The plant material originated from a commercial nursery in Franconia, Germany, and consisted of three-year-old trees maintained under uniform conditions prior to the experiment.The experiment aimed to assess the physiological responses of P. menziesii to progressive drought and subsequent recovery under controlled environmental conditions. Two drought treatments (mild and severe) were applied over a four-week period, followed by a re-watering phase. Air and soil temperature, relative humidity, vapor pressure deficit, molar flow, transpiration rate, net photosynthesis, conductance to water, and CO₂ exchange were recorded continuously using automated LI-COR gas exchange systems with separate branch (aboveground) and root (belowground) chambers.Each measurement is associated with a unique tree identifier, treatment level, and compartment. All timestamps are reported in Coordinated Universal Time (UTC). The dataset provides detailed observations suitable for examining drought stress responses and recovery dynamics in juvenile Pseudotsuga menziesii under controlled laboratory conditions.