Subcellular mapping of gold in cancer cells treated with gold(III)-based anticancer drug candidates and correlation with organelle fluoresce

DOI

A wide range of molecular gold compounds display cytotoxic properties and may in turn provide safer and more efficient alternatives to clinically approved platinum based anticancer drugs. In this context, structure-activity relationship studies we performed on an array of cationic biphenyl gold(III) complexes with a carbene-pyridine chelating ligand showed a marked dependence of both the antiproliferative property and the chemical reactivity on the nature of the C^N ligand. We now wish to examine by nano-XRF the impact of the C^N ligand of 3 representative gold complexes on the distribution and concentration of gold in cancer cells shortly exposed to each of these complexes at biologically meaningful concentration. Correlative fluorescence microscopy of cellular organelles with multicolour staining dyes will warrant precise location of gold and other heavy elements in cryo-fixed cancer cells

Identifier
DOI https://doi.org/10.15151/ESRF-ES-2051955633
Metadata Access https://icatplus.esrf.fr/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatplus.esrf.fr:inv/2051955633
Provenance
Creator Sylvain BOHIC ORCID logo; Joelle SOBCZAK ORCID logo; Michele SALMAIN ORCID logo; Tom LACOMA; BENOIT BERTRAND ORCID logo
Publisher ESRF (European Synchrotron Radiation Facility)
Publication Year 2028
Rights CC-BY-4.0; https://creativecommons.org/licenses/by/4.0
OpenAccess true
Representation
Resource Type Data from large facility measurement; Collection
Discipline Particles, Nuclei and Fields