Context and methodology

This data was collected within the framework of the PhD thesis by Anna Schmidbauer. The thesis has been published in TU Wien's reposiTUm as referenced below. The data is currently restricted as it also contains data which might be subject to further exploitation; however, it will be published in separate, public entries as soon as possible. The thesis aimed for the development of biointeractive hydrogels, which potentially improve the integration of artificial 3D-printed bone scaffolds. The central concept involvedincorporating growth factors or bioactive molecules into these scaffolds to potentially boost bone regeneration and enhance osseointegration. Therefore, bio-based materials were chosen as starting materials due for their potential to closely mimic natural bone characteristics. The first part of this work focused on hydrogels derived from decellularized bone lysate (BL), leveraging the effectiveness of homologous tissues over heterologous scaffolds. The second part aimed for hydrogels based on human platelet lysate (PL) as the main building block, known for its remarkable regenerative potential regarding biointegration and wound healing.   

Technical details

The data set is organized in several files/folders as described below.

PDF file "Laborjournal_Schmidbauer" contains a full scan of the lab notebook.

XLSX file "substances_thesis_Anna Schmidbauer" contains the substances used and synthesized during the thesis.

XLSX file "raw_data_figures" contains data sets of all figures from the thesis.

PPTX and OPJU files "Figures_thesis_Anna Schmidbauer" contain processed/visualized data.

Folder "Raw Data" contains the following subfolders (as ZIP) organized by the methodologies used for acquisition. (in alphabetical order)

"DLS data" contains CSV data from dynamic light scattering measurements of the used platelet lysate (PL) sample

"In vitro data" contains CSV data from measurements of "BSA assay", "degradation", "rheology", and CZI files (Zeiss microscopy image raw files) and TIF renders of LSM (laser scanning microscopy) of cells encapsulated in hydrogels after 1, 3 and 9 days in "LSM images" with measured fluorescence intensity in the XLSX file "Fluorescence".

"In vivo data" contains JPG renders (with added scale bars), raw JPG and VSI files (Olympus microscopy image raw files) of histological examinations in "Histo", macroscopic images of test subjects at different time points as JPG in "subject images", and compilations of the two different hydrogel types (PLAGE and PLGMA) as PPTX files

"MALDI data" contains Matrix Assisted Laser Desorption Ionisation mass spec data as CSV and compiled as OPJU file

"NMR data" contains raw NMR data of substances referenced in the PDF file "Laborjournal_Schmidbauer".

"Photorheology data" contains photorheological measurements of hydrogel formulations, based on precursors as indicated in the folder name as CSV and XLSX, respectively.

"SDS PAGE" contains scans of electrophoresis experiments as SCN files (BioRad scan raw file) and PNG/JPG/TIF render files, Bradford assay evaluations as XLSX files, and a results compilation as PPTX file.

File types:

opju (OriginLab Origin project): created with OriginPro 2023b (64-bit) SR1 10.0.5.157 (Lehre)  

.RhPrj (Anton Paar RheoCompass, shown as ZIP internally): processable with Anton Paar RheoCompass Software

.czi (Zeiss microscopy images): processable with ZEISS ZEN lite software

.vsi (Olympus microscopy images): processable with Olympus Stream Software

.scn (BioRad electrophoresis scans): processable with BioRad Image Lab Software 

Bruker NMR raw data directories (pdata, acqu, shimvalues, etc.): processable with MestreNova or Bruker TopSpin