Nanostructured lipid aggregates offer enormous potential as tools for membrane structural biology and biophysics. Poly(Styrene-Maleic Acid) stabilized lipid nanodiscs can be easily prepared, without detergent, at low cost and can solubilize membrane proteins. In this proposal we wish to study the adsorption of polymer-stabilised lipid nanodiscs containing a membrane protein at a lipid monolayer containing a lipid with a His-tag chelating ligand on both solid-liquid & air-liquid interfaces. We wish to determine whether the protein containing discs can be close packed in a single orientation at the interface to assist in future structure determination & potentially bulk 3D crystalisation. We will use ZipA, a membrane protein involved in cell division, with a single transmembrane helix (in collaboration with Prof Dafforn's group in Birmingham) for this proof-of-principle experiment.