The data presented derive from sediment core MD99-2203, collected in 1999 off Cape Hatteras as part of the IMAGES project. The 41-m-long sedimentary sequence was retrieved during leg MD114 by the R/V Marion Dufresne, equipped with a Giant Calypso corer. The primary objective was to document paleoceanographic and paleoclimatic changes at 35°N in the southwestern North Atlantic, at the boundary between subtropical and subpolar gyre influences, over the past 50,000 years, .We present pollen raw data from the samples analyzed between 1350 and 3800 cm depth (corrected depth: 1226-3672 cm). Most of the pollen analysis was performed at 10 to 20 cm resolution. The sampling resolution in the intervals 1910-2560 cm (corrected depth: 1786-2436 cm) and 3138-3800 cm (corrected depth: 3014-3672 cm) varies between 17 and 40 cm. One hundred thirty samples were analyzed for pollen grains and spores. Sample preparation followed standard palynological protocols using HCl and HF. For each sample, 1.5-5.5 cm³ of fine sediment (<150 µm) was processed at an average sampling resolution of 10–20 cm. The final residues were mounted unstained in bidistilled glycerin, a mobile mounting medium that allows rotation of pollen grains and spores.Pollen and spores were identified using a Zeiss Axio Imager A10 light microscope at 400X and 1000x magnifications. In most of the samples, a minimum of 100 pollen grains, excluding Pinus, and 20 pollen morphotypes were counted.