Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013

For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies).

Identifier
DOI https://doi.org/10.1594/PANGAEA.835180
PID https://hdl.handle.net/10013/epic.43161
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.835181
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.835180
Provenance
Creator Schmithüsen, Tosia; Niehoff, Barbara ORCID logo; Auel, Holger
Publisher PANGAEA
Publication Year 2014
Funding Reference Seventh Framework Programme https://doi.org/10.13039/100011102 Crossref Funder ID 264933 https://cordis.europa.eu/project/id/264933 Basin Scale Analysis, Synthesis and Integration
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 126 data points
Discipline Earth System Research
Spatial Coverage (-56.074W, 56.446S, -10.869E, 62.819N); North Atlantic Ocean
Temporal Coverage Begin 2013-03-25T13:24:00Z
Temporal Coverage End 2013-04-11T10:43:00Z