The Great Barrier Reef (GBR), Australia, supports over 1200 species of fish and is the largest coral reef ecosystem in the world, comprising 2500 reefs across approximately 344,400 km2. Despite the global importance of the GBR, little is known about the natural diversity of viruses that infect tropical reef fish, as well as the ecological and evolutionary processes that allow such viruses to spread within a reef fish community. To this end, we employed metatranscriptomic sequencing to characterize the viromes of 61 tropical reef fish species, including those occupying a 100 m2 reef fish community.