Dataset related to the characterization of formate and glycerol dehydrogenases, reaction medium engineering, and optimization of the multi-enzymatic production of formate and DHA from industrial waste such as CO2 and glycerol. Additionally, the evaluation of the reaction under industrially relevant conditions using real industrial waste was included.

Data of the simultaneous production of formate and DHA by a multi-enzymatic system for the valorization of industrial waste: CO2 and glycerol.

Description of the methods used to collect and generate the data: The enzymatic activity of the enzymes was measured spectrophotometrically at 340 nm using a phosphate buffer, NAD+ at 1.67 and 5 mM, 100 mM sodium formate, 100 mM glycerol, and a variable amount of soluble enzyme. The protein concentration was quantified spectrophotometrically at 595 nm using the Bradford method. The concentration of soluble CO2 in the medium was measured using a CO2 sensor (InPro5000i/12/220, Mettler Toledo S.A.E). Formate, DHA and glycerol quantification were performed in the liquid chromatograph using an ion exchange method with the IC-Sep COREGEL 87H3 column and sulfuric acid (H2SO4) 0.5 mM: Acetonitrile (65:35) as mobile phase. A flow rate of 0.6 mL·min-1, injection volume of 20 µL, column temperature of 30 °C, UV/Visible detector at 210 nm and RID detector at 30 °C. The multi-enzymatic production of formate and DHA was carried out in 250 mM phosphate buffer at pH 8.5, pre-saturated with a gas mixture containing 24% CO2 in nitrogen, with 1 mM NADH, 0.5 U mL⁻¹ FDH, 3.5 U mL⁻¹ GlyDH, and 100 mM glycerol. The reaction was performed for 30 hours at 30°C with constant stirring. Information about instruments, calibration and standards: Varian Cary 50 Bio UV-visible Spectrophotometer (Agilent) SPECTROstar Nano (BMG LABTECH) Spectrophotometer Fast Protein Liquid Chromatography (FPLC) in ÄKTA Pure (GE Healthcare®, Chicago, IL, USA) Liquid chromatograph Agilent 1220 Infinity II Multi Therm Heat Block system (Benchmark Scientific Inc.). Environmental or experimental conditions: The reactions were carried out in a fume hood at 30°C and within a pH range of 7.0 to 8.5.