This dataset contains the IgG antibody profile as measured using a peptide microarrays containing 3,760 linear B-cell epitopes from 196 human pathogens from 18 patients with pediatric uveitis (18 serum samples and 17 paired aqueous humor samples) and 6 age-matched controls (6 serum samples and 5 paired aqueous humor samples).

This dataverseNL dataset contains the original tif. files from the microarray scans, raw data, processed data, data results and metadata (see readme file and R notebooks (R script and Image) used for the analysis in the manuscript:

Hendrikse et al. Pediatric autoimmune uveitis is associated with intraocular antibodies against Epstein-Barr virus Nuclear Antigen 1 (EBNA-1).

Raw IgG intensity data (all samples combined and calculated mean per peptide) "Raw_data_microarrays.csv"

R scripts with step by step analysis

'Statistical analysis' (see Analysis.html)

'Figure 1' (see "Figure_1.html")

'Figure 2 and 3' (see "Figure-2_3.html")

Infectious disease peptide annotation map "Peptide_Map_Infectious_Disease_Epitope_Microarray.csv"

Deep profiling of the antibody-repertoire of patients with non-infectious uveitis may reveal a distinct natural history of microbial exposure that may increase the susceptibility to non-infectious uveitis. This may be reflected by enrichment for IgG antibodies towards distinct pathogenic species or antibody epitope motifs. We used peptide microarrays containing 3,760 linear B-cell epitopes from 196 human pathogens to profile IgG levels in eye fluid biopsies and paired serum samples from 18 Dutch pediatric patients and 6 age-matched controls. We compared intensities of single epitopes and clusters based on overlapping amino acid sequence of peptides. Next-generation sequencing data was obtained to determine the HLA-DRB115:01 genotype. Intraocular antibodies against an immunogenic epitope of EBV associated with HLA-DRB115:01 is implicated in pediatric uveitis and suggests altered host immune responses to EBV infection in autoimmune uveitis.