This study examines the colonization and dynamics of microbial composition on different plastic polymers (PE, PP, PS, PET) and non-plastic surfaces (glass and wood) in a semi-sterile seawater aquarium filled with marine plastic debris. We tested the water in the aquarium and any type of surface after 2-90 days. We aimed to understand the transition of the bacteria population from plastic debris to new surfaces, observe the changes in microbial composition from early to mature biofilm stages and identify bacteria with preferences for plastic surfaces. To achieve this, we employed high-throughput sequencing technology- Nanopore MinION.