This dataset is a component of the PAN-Arctic data collection of benthic BIOtas (PANABIO) and has been collated during the Census of Marine Life project Arctic Ocean Diversity (ArcOD; http://www.arcodiv.org/). It contains a total of 221 presence records of 34 epibenthic megafaunal taxa (species to genus level) identified in seabed images taken at 25 stations located in western Fram Strait off NE Greenland at water depths of 27–375 m during a cruise in 1993. The dataset is also available in a PostgreSQL-based data warehouse that can be accessed and queried through an open-access frontend web service at https://critterbase.awi.de/panabio.