To reduce environmental pollution and pesticide residues, an increasing number of fungi are being used in biological control. Chaetomium globosum is a fungus found in plants, soil, straw, and manure, and the strain was isolated from red algae. Genomic DNA was sequenced using dual-end Illumina technology, resulting in the complete genome sequence of Chaetomium globosum, which is 33,107,708 base pairs in length and contains 8,704 annotated protein-coding genes. This genomic information provides a valuable reference for the application of Chaetomium globosum in biological control.