Replication Data for: Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size

DOI

Raw data of research article "Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size" The objective of this study was to assess the effect of CAPA-IVM on oocyte competence of prepubertal goat oocytes derived from small ( 3 mm) follicles. In experiment 1, oocytes were cultured for 6 h in pre-IVM medium with the meiotic inhibitors CNP and estradiol (CNP group) or without them (control group), and GV rate and chromatin configuration were assessed. In experiment 2, oocytes were cultured in conventional IVM (c-IVM) or CAPA-IVM (pre-IVM followed by c-IVM) and blastocyst development, epidermal growth factor receptor (EGFR) protein expression in oocytes and cumulus cells (CCs), and intra-oocyte ROS and glutathione (GSH) level were assessed.

Dades brutes de l'article de recerca "Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size" L'objectiu d'aquest estudi va ser avaluar l'efecte de CAPA-IVM sobre la competència ovocitària dels oòcits de cabra prepuberals derivats de fol·licles petits ( 3 mm). En l'experiment 1, es van cultivar oòcits durant 6 h en medi pre-IVM amb els inhibidors meiòtics CNP i estradiol (grup CNP) o sense ells (grup control), i es va avaluar la taxa de GV i la configuració de la cromatina. En l'experiment 2, es van cultivar oòcits en IVM convencional (c-IVM) o CAPA-IVM (pre-IVM seguida de c-IVM) i desenvolupament de blastocist, expressió de proteïnes receptores del factor de creixement epidèrmic (EGFR) en oòcits i cèl·lules cúmuls (CC), i es va avaluar el nivell intraoòcit de ROS i glutatió (GSH).

Identifier
DOI https://doi.org/10.34810/data1389
Metadata Access https://dataverse.csuc.cat/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34810/data1389
Provenance
Creator Ferrer Roda, Mònica (ORCID: 0000-0003-3069-435X); Paramio Nieto, Maria Teresa (ORCID: 0000-0002-3653-654X); Izquierdo Tugas, Dolors ORCID logo
Publisher CORA.Repositori de Dades de Recerca
Contributor Izquierdo Tugas, Dolors; Universitat Autònoma Barcelona
Publication Year 2024
Funding Reference Spanish Ministry of Science, Innovation and Universities PID2020-113266RB-100 ; Generalitat of Catalunya AGAUR 2020 FI-B 00025
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Izquierdo Tugas, Dolors (Universitat Autònoma de Barcelona)
Representation
Resource Type Experimental data; Dataset
Format application/x-rar-compressed; text/tab-separated-values; text/plain
Size 6865363; 301; 1192164474; 4839; 523; 82841375; 3409; 720; 11141; 260541316; 3026
Version 2.3
Discipline Agricultural Sciences; Agriculture, Forestry, Horticulture, Aquaculture; Agriculture, Forestry, Horticulture, Aquaculture and Veterinary Medicine; Life Sciences; Medicine