The ecotoxicological effects of heavy metal combined pollution are still in the preliminary stage. The project "Study on the regulatory role and Mechanism of gut microbes in Response to the web formation Function of spiders in Farmland to heavy metal Cd and Pb combined pollution" integrates multi-disciplinary theories, combines field investigation and laboratory tests from the perspective of animal behavior, and utilizes a variety of technologies and project methods to regulate protein anabism from the perspective of gut microbes. To explore the regulation and mechanism of intestinal microorganisms on the response of spider web forming function to heavy metal Cd and Pb combined pollution. The project consolidates and innovates the response mechanism of spider web building function in farmland to heavy metal composite pollution. At the same time, the regulation of the effect of intestinal microbiome - host interaction on heavy metal toxicity was enriched and developed. It also provides a new research idea for the functional gene mining of spider gut microbes. This project provides theoretical and practical support for exploring the ecotoxicological effects of heavy metal combined pollution in farmland, and also provides a new research perspective for the web-building function of farmland spiders and the protection and utilization of predatory natural enemies.