We explored the effects of ocean acidification on marine benthic communities using DNA metabarcoding of COI to assess the diversity of algae and metazoans. Specifically, we examined the natural pH gradient generated by the Fuencaliente CO2 vent system, located near La Palma Island (Canary Islands). Analyses revealed high levels of taxonomic diversity in an acidified natural area for the first time.</p><p>Files are multiplexed files that can be demultiplexed using obitools ngsfilter command. The ngsfilter tables with the sample tags for each sample are publicly available in https://github.com/Sgonzalezdelgado/MetabarcodingCO2vent/tree/main/ngsfilter_files