Measurements of particulate organic carbon and nitrogen concentrations, extracellular polymeric substance concentrations and flow cytometry measurements from an aggregation experiment in a sub-Arctic fjord, Ramfjord in Tromsø, Norway

DOI

Fjord water was collected on a monthly basis between September 2020 and August 2021 from 5m in a sub-Arctic fjord (Ramfjorden, Tromso, Norway) with GoFlos for aggregation experiments and filtered over a 90mum mesh to remove large grazers. Part of the water was filtered through a GFF filter (pore size 0.7mum, Whatman), while another part was left unfiltered. Samples for particulate organic carbon (POC) and particulate nitrogen (PN), extracellular polymeric substances (EPS), and flow cytometry were taken before the water was filled into self-manufactured roller tanks (time0). 8 tanks were rolled on a rolling table at 3rpm (5 tanks filtered, 3 tanks unfiltered water) while 4 tanks were left standing (2 tanks filtered, 2 tanks unfiltered) for 36 hours. The same parameters were sampled again after the incubation (time1). Water for POC/PN analyses (~500-1500ml) was filtered through a GFF filter (0.7mum, Whatman), and for EPS (~150ml) through a polycarbonate filter (0.4mum, Whatman) in triplicates at time0, and in singlets from each tank at time1. POC/PN and EPS samples were frozen and stored at -20C until further processing. Samples for flow cytometry (5ml) were fixed with glutaraldehyde (0.5% final concentration) at time0, and at time1 from each tank and frozen at -80C. Upon processing, the filters forPOC/PN were dried, acidified and again dried before they were analyzed with an elemental analyzer (Exeter Analytical CE440). EPS were colorimetrically determined following the method of Dubois et al. (1956) and the absorbance at 485nm was measured with a spectrophotometer (UV-6300PC; VWR). Bacteria, viruses, pico- and nano-sized phytoplankton were counted with an Attune Flow Cytometer (Applied Biosystems, Life Technologies).

Identifier
DOI https://doi.org/10.11582/2024.00121
Metadata Access https://search-api.web.sigma2.no/norstore-archive/oai/v1.0?verb=GetRecord&metadataPrefix=oai_dc&identifier=doi:10.11582/2024.00121
Provenance
Creator Bodur, Yasemin V.; Digernes, Maria Guadalupe; Lund Paulsen, Maria; Dubourg, Paul; Dietrich, Ulrike
Publisher Norstore Archive
Contributor Department of Geosciences, University of Oslo, Norway, UiO
Publication Year 2024
OpenAccess true
Contact Norstore Archive
Representation
Language English
Resource Type Dataset
Discipline Biology; Chemistry; Life Sciences; Natural Sciences