Phytoplankton pigment concentrations measured by HPLC in the surface water of the Mackenzie Delta Region during 4 expeditions from spring to fall in 2019


The determination of phytoplankton using high performance liquid chromatography (HPLC) is detailed in Hooker et al. (2005; doi:10.1364/AO.44.000553) and Ras et al. (2008; doi:10.5194/bg-5-353-2008). Briefly, the pigments of particles retained on GF/F (0.7 µm) filters were extracted at -20°C with 3 mL methanol (100%). The filter samples were then disrupted using a sonicator and clarified one hour later by vacuum filtration through GF/F filters. The extracts were rapidly analyzed within 24h by HPLC with a complete Agilent Technologies system (comprising LC Chemstation software, a degasser, a binary pump, a refrigerated autosampler, a column thermostat and a diode array detector).

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Creator Matsuoka, Atsushi ; Juhls, Bennet ; Bécu, Guislain ; Oziel, Laurent ; Leymarie, Edouard; Lizotte, Martine ; Ferland, Joannie; Doxaran, David ; Maury, Juliette; Béguin, Marine; Laberge-Carignan, Audrey; Guilmette, Caroline; Hilborn, Andrea; Tisserand, Lucas; Devred, Emmanuel; Bossé-Demers, Thomas; Mannino, Antonio; Babin, Marcel
Publisher PANGAEA
Publication Year 2021
Funding Reference Horizon 2020, 773421
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 4635 data points
Discipline Biogeochemistry; Biospheric Sciences; Geosciences; Natural Sciences
Spatial Coverage (-138.135W, 68.264S, -133.031E, 69.649N); Mackenzie Delta, Canada
Temporal Coverage Begin 2019-04-20T15:34:00Z
Temporal Coverage End 2019-09-08T12:00:00Z