Wire and Arc Additive Manufacturing (WAAM) process is rapidly moving towards the production and qualification of large structural parts. Parts built by WAAM for structural applications require to perform under cyclic loading condition without compromising the safety. The presence of heterogeneous microstructure and considerable texture leads to anisotropic mechanical properties. More importantly, the microstructure heterogeneity leads to direction dependent deformation and damage under cyclic loading. Therefore, the proposed study aims to understand the influence of two deposition strategies and the microstructure heterogeneity in WAAM Ti6Al4V specimens subjected to low cycle fatigue. The outcomes will provide a mechanistic understanding of the relation between microstructure heterogeneity on the deformation and twinning in WAAM Ti6Al4V.