Data set shows on one side the signalment, clinical data, clinical stage of leishmaniosis according to LeishVet guidelines, various serum and urinary parameters including urinary amylase to creatinine ratio, and urinary electrophoresis in dogs with leishmaniosis at the time of diagnosis and after anti-Leishmania conventional treatment and on the other the signalment, clinical data, various serum and urinary parameters including urinary amylase to creatinine ratio in healthy dogs to compare dogs with leishmaniosis at the time of diagnosis with healthy dogs and to compare dogs with leishmaniosis at different stages of the disease.