Validation of mouse model: qPCR and Western blots probing for Tor1a-torsinA

DOI

Model validation dataset associated with Pocratsky et al 2023, Science Translational Medicine

Data generated from postnatal day 18 littermate control (Cdx2::wt;Tor1a-wt/frt) and spinal Tor1a d-cko mice (Cdx2::FlpO;Tor1a-frt/frt) that develop early onset generalised torsional dystonia. Whole tissues were snap frozen and processed for expression of Tor1a and torsinA protein. Raw images of Western blots provided

Validation_qPCR.xlsx - dataset: Tor1a expression in brains and lumbar spinal cords

Validation_Westerns.xlsx - dataset: torsinA expression in brains and lumbar spinal cords

Western_metadata: details for attached images of blots

Full methodological detail provided in manuscript

Identifier
DOI https://doi.org/10.5522/04/19642395.v1
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Metadata Access https://api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:figshare.com:article/19642395
Provenance
Creator Pocratsky, Amanda; Do Nascimento Xavier Fernandes, Filipe; Ozyurt, Gorkem; White, Ian; Sullivan, Roisin; O'Callaghan, Benjamin; Smith, Calvin; Surana, Sunaina; Beato, Marco; Brownstone, Rob
Publisher University College London UCL
Contributor Figshare
Publication Year 2023
Rights https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact researchdatarepository(at)ucl.ac.uk
Representation
Language English
Resource Type Dataset
Discipline Life Sciences; Medicine; Neurosciences