This collection contains raw mass spectrometry data for the metabolomics measurements published in the manuscript: "Deciphering the Physiological Response of Escherichia coli Under High ATP Demand" (https://doi.org/10.15252/msb.202110504) by Simon Boecker, Giulia Slaviero, Thorben Schramm, Witold Szymanski, Ralf Steuer, Hannes Link, Steffen Klamt (klamt@mpi-magdeburg.mpg.de). The subcollection "Raw data" contains all data as .txt files and the excel file maps the data sets to the different strains.