RNA-seq analysis of kinase deletion mutant (?mpkA) and parental strain Aspergillus nidulans reveal novel cellular functions of MpkA.

A transcriptomic assessment of a kinase deletion mutant (?mpkA) and a control strain revealed many cellular activities this kinase is impacting. Studies were conducted in the model filamentous fungi, Aspergillus nidulans¸ under rich growth conditions. MpkA is a known kinase involved in the cell wall integrity signaling pathway. This pathway is activated upon cell wall stress and regulates production of cell wall related genes. Here, fungi were grown under non-stressful conditions. The comparison between the control strain and the deletion mutant transcript levels revealed previously unknown functions, such as iron starvation, that MpkA may be involved with. Overall design: A control strain and deletion mutant grown in non-stressful conditions (2 biological replicates and 2 technical replicates) were analyzed to determine alternative, unknown functions of MpkA using RNA-seq.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0127226CC407EE81378BF8579E633BD30A603C224CF
Metadata Access https://data.blue-cloud.org/api/collections/7226CC407EE81378BF8579E633BD30A603C224CF
Provenance
Instrument Illumina Genome Analyzer IIx; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2019-05-03T00:00:00Z