Small angle neutron diffraction study of water re-crystallisation in solutions used for cryo-preservation of complex biological objects

DOI

Cryo-preservation is one of the most efficient methods used for storage and recovery of endangered species tissue for fertility treatment. Under low temperature conditions (~ 80K) the cells of plants and animals can be preserved in a viable state for many years. The major obstacle on a way to successful cryopreservation is the re-crystallization of water in successfully vitrified biological samples during warming-up process. We propose a neutron scattering investigation of the re-crystallization process in vitrified sample during warming up. Experimentally we are planning to study cryo-protectant solutions sample used in our previous experiment on diffractometer SANDALS. The main aim of the experiment is to determine a phase diagram and find a temperature range where re-crystallization takes place.

Identifier
DOI https://doi.org/10.5286/ISIS.E.42581165
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/42581165
Provenance
Creator Dr Borys Dzyuba; Dr Oleg Kirichek; Professor Alan Soper; Mr Sergii Boryshpolets; Professor Barry Fuller; Dr Sam Callear
Publisher ISIS Neutron and Muon Source
Publication Year 2016
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2013-12-06T00:00:00Z
Temporal Coverage End 2013-12-11T00:00:00Z