Raw data from a series of experiments aimed to evaluate the feasibility of producing lipopeptides in solid-state fermentation using thermofiles.

Universitat Autònoma de Barcelona. Grup de Recerca en Compostatge (GICOM).

This study conducted solid-state fermentation (SSF) using Bacillus subtilis in SSF reactors, with optimized conditions lipopeptides (LPc) production. LPc yield was quantified through water extractions and gravimetric meassure.

1. Description of methods used for collection-generation of data:

Lab scale experiments were carried out using 24 0.5-L reactors, 12 erlenmayers 0f 500ml, and a isolated reactor of 50L.

1. Methods for processing the data:

2. LPc-crude was analytically determined after water extraction.

3. Lipopeptides congeners identified by UHPLC-ESI-MS

4. Instrument- or software- specific information needed to interpret the data:

5. Excel or similar software.

6. Instruments, calibration and standards information:

7. Analytical balance Sartorius Entris II

8. Orbital shaker (Infors HT, UK)

9. HPLC UltiMateTM 3000

10. Environmental or experimental conditions:

11. Bacillus subtilis CBI-7S1 was cryopreserved at -80°C.

12. Microorganism growing conditions were set up at 50 ºC, 180 rpm at 48 h.

13. 0.5-L bioreactors operational parameters were established at: temperature 30, 40 , 50, 60 ºC, humidefied airflow rate 30 mL/min.

14. Quality-assurance procedures performed on the data:

All temperature experiments were carried out using 3 reactors. For each DOE condition 1 reactor was used.