Lamellibrachia luymesi dominate cold sulfide-hydrocarbon seeps, and are known for their ability to consume bacteria for energy. The symbiotic relationship between tubeworm and bacteria with special adaptations to chemosynthetic environments has received attention. However, metabolic studies have primarily focused on mechanisms and pathways of bacterial partner in symbiosis, and studies from the animal host are limited. Therefore, we further investigate adaptation to deep-sea chemosynthetic environments in L. luymesi.