In this study, we observed a methane depression phenomenon induced by the accumulation of fire-derived pyrogenic carbon in peat soil. This observation was obtained through laboratory microcosm and bioelectrochemical incubation experiments. The measured parameters involved in this observation were mainly methane production rate, environmental electron transfer balance, and isotopic tracking that shows the degradation extent of pyrogenic carbon. The data was collected from 2017 to 2018 during the laboratory incubation of peat soil from New York State, USA. We collected the data to investigate the effect of pyrogenic carbon in controlling greenhouse gas emissions in peat soils. The data was collected by carbon isotopic analysis system, electron transfer monitoring and quantification device, and microbial sequencing analysis.