Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2022 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu in the Christian-Albrechts-Universität zu Kiel at 10 °C. For experimental characterization of carbohydrate digestion between September and November 2023, S. purpuratus larval cultures were maintained with two different food conditions (low food vs. high food) under low light conditions, following a 12-hour dark to 12-hour light cycle at 15°C, with continuous mixing facilitated by a gentle stream of pressurized air bubbles. Health assessments and larval size were conducted by sampling two 10 ml water samples at 0, 3, 5, 7, 9 & 10 days larval age.

The attached tables contain the data collected in laboratory experiments at the Christian Albrechts University, Kiel between September and November 2023. Larvae of cultured S. purpuratus were cultured for up to 10 days for the data collection. Several F1 generations were generated for the experiments, which were treated independently of each other. The larval test organisms were cultivated in climate chambers at principally 31.5 psu, 15.5 °C and pH 8.2. ----Experimental food treatments: Low food: 500 cells Rhodomonas sp. used / ml total culture High food: 8000 cells Rhodomonas sp. used / ml total culture