Breast cancer (BC) often metastasizes to bone. Bone tissue is highly dynamic and the cellular and structural microenvironment are strongly interlinked. Mineralized tissue is highly vascularized and rich in osteocytes, highly inter-connected via their osteocyte-lacunar canalicular network (LCN), cell dendrites of few hundreds of nm. Alterations in osteocytes and LCN characteristics have been associated to cancer. With our mouse model of early bone metastasis, we could detect BC cells and small clusters using 3D light sheet fluorescence microscopy (LSFM) and track early bone lesion growth with time-lapse in vivo µCT. Since the LCN plays a fundamental role in cell–cell signaling and bone remodeling, we aim to examine the LCN topology at early stages of metastasis. With synchrotron nanoCT on ID16B we aim to characterize LCN topology as a function of distance to metastatic lesions and BC cells, and provide new insights in the structural and temporal changes during early bone metastasis.