The dataset contains measurements of organic biomarkers and sterols, dinoflagellate cyst census counts, and benthic and planktonic stable oxygen isotope data from core GS16-204-22CC-B on the Eirik Drift (58°02.83′N, 47°02.36′W; 3160 m water depth) for Marine Isotope Stage 6, 5e and 5d. Total Organic Carbon (TOC), the Highly Branched Isoprenoids (HBIs) IP25, HBI II, HBI III (Z), and HBI III (E), and the sterols brassicasterol, dinosterol, campesterol and sitosterol were measured in the organic geochemistry lab at the Alfred Wegener Institute, Bremerhaven, Germany. Biomarker values are presented as biomarker/g dry sediment and biomarker/g TOC. Dinoflagellate cysts were counted along non overlapping traverses at x40 magnification using a transmitted light microscope and classified to species level as much as possible. The dataset consists of raw counts, relative abundances and dinocyst concentrations (cysts/g dry sediment). Stable oxygen isotopes (δ18O) were measured on the planktic foraminifera Neogloboquadrina pachyderma (sinistral) and benthic foraminifera Cibicidoides wuellestorfi. Values are reported relative to Vienna Pee Dee Belemnite. The measurements were carried out at FARLAB, University of Bergen, Norway. The age model for core GS16-204-22CC-B was obtained by correlation of benthic and planktic δ18O measured in this core with previously published isotope records in Irvali et al. (2012; 2016) and a 14C age from Griem et al. (2019).