This dataset includes confocal microscopy images, flow cytometry data, dynamic light scattering (DLS) measurements, and cryo-transmission electron microscopy (Cryo-TEM) data related to the study of cell-derived vesicles (CSMs) from eukaryotic and prokaryotic origins. The dataset also contains experimental results on the thermal stability, size distribution, and interaction of CSMs with homologous cells. The dataset aims to contribute to the understanding of vesicle-based drug delivery systems and their potential biomedical applications.

METHODOLOGICAL INFORMATION

Description of methods used for collection-generation of data: Data was collected using confocal microscopy, flow cytometry, DLS, and cryo-TEM for the characterization of cell-derived vesicles from HeLa, SH-SY5Y, and E. coli cells. The membranes were extruded using 400 nm and 100 nm filters to generate the vesicles, and the experiments focused on their thermal stability, interaction with cells, and size distribution.

Methods for processing the data: Data was processed using CytExpert for flow cytometry analysis and Zetasizer Nano ZS for DLS analysis. ImageJ was used for analyzing confocal microscopy and Cryo-TEM images.

Instrument- or software-specific information needed to interpret the data: CytExpert Software (Beckman Coulter) Zetasizer Nano ZS (Malvern Panalytical) ImageJ for image processing ZEISS LSM 980 confocal laser scanning microscope

Instruments, calibration and standards information: Instruments were calibrated according to the manufacturer's instructions, with regular maintenance and quality assurance performed before experiments.

Environmental or experimental conditions: Confocal microscopy was conducted at 37°C with 5% CO₂, and DLS measurements were carried out at temperatures ranging from 4°C to 70°C.

Quality-assurance procedures performed on the data: Triplicate measurements were taken for DLS analysis to ensure accuracy and reproducibility of the data. Controls were included in all microscopy and flow cytometry experiments.