For most chemicals, environmental toxicity is only investigated for a single generation. In this study we investigate the effect of the fungicide prochloraz across generations in Daphnia magna. The study design included two exposure scenarios one where all three generations were continuously exposed to prochloraz (100 ug/L) and one where only the first generation (F0) was exposed. We studied effects at different levels of biological organization combining key phenotypic effects, such as growth and reproduction, CYP enzyme activity, metabolomics and for generation F2 also transcriptomics