Amphipod glycogen and glucose content under simulated invasion stress in wetlands

DOI

Every two weeks during the 6-week acclimation period, 8 adults were randomly selected from each tank glycogen analysis (n = 8). Individuals were sacrificed in dry ice and stored at -80 °C. Frozen amphipods were ground into fine powder using a liquid nitrogen cooled mortar and pestle. Glucose and glycogen metabolites were extracted separately to be analyzed using a colorimetric assay. Glycogen was extracted by adding 1 ml of ice-cold 8% HClO₄ to approximately 20 mg of powdered amphipod tissue and then homogenized on ice for approximately 10 seconds with a Pro200 Bio-Gen Series homogenizer (PROScientific, Oxford, CT, USA). A 200 μl sample of the homogenate was frozen at -80 °C for later glycogen analysis. The remaining homogenate slurry was centrifuged at 10,000 g for 10 minutes at 4 °C and the supernatant was extracted and neutralized with 3 mol l⁻¹ K₂CO₃. The solution was centrifuged again at 10,000 g for 10 minutes at 4 °C and frozen at -80 °C for the glucose assay. Glycogen samples were enzymatically digested using previous methods (Hassid & Abraham, 1957) and analyzed for glucose following methodology (Bergmeyer, 1983) modified for microplate spectrophotometer (Synergy HT, Biotek, Winooski, VT, USA). Glycogen content was then corrected for starting glucose.

Funding:* Community function under Phragmites invasion in Suisun Marsh (https://www.seacrlab.com/), Award: Delta Stewardship Council DSF R/SF-107

Identifier
DOI https://doi.org/10.1594/PANGAEA.973189
Related Identifier IsPartOf https://doi.org/10.1594/PANGAEA.973173
Related Identifier References https://doi.org/10.1016/S0076-6879(57)03345-5
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.973189
Provenance
Creator Tanner, Richelle L ORCID logo; Haworth, Lorna; Nancollas, Sarah; Landa, Susie; Todgham, Anne E
Publisher PANGAEA
Publication Year 2024
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 2794 data points
Discipline Biospheric Sciences; Ecology; Geosciences; Life Sciences; Medicine; Medicine and Health; Natural Sciences; Physiology
Spatial Coverage (-121.983 LON, 38.118 LAT); Suisun Marsh, California, United States