This dataset is a component of the PAN-Arctic data collection of benthic BIOtas (PANABIO) and has been collated during the Census of Marine Life project Arctic Ocean Diversity (ArcOD; http://www.arcodiv.org/). It contains a total of 597 wet biomass records of 103 megabenthic taxa (species to genus level) identified in 29 beam trawl catches taken at 29 stations located in the Chukchi and eastern Bering Seas at water depths of 26–101 m during three cruises between 2004 and 2007. The dataset is also available in a PostgreSQL-based data warehouse that can be accessed and queried through an open-access frontend web service at https://critterbase.awi.de/panabio.