In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation. Overall design: Global transcriptional analysis in the wild type strain (biA1) grown under four different nitrogen and carbon regimes. RNA was isolated from three biological replicates grown under the same conditions and combined in one sample for RNA-seq.