Artemia is not only one of the most important live feed for larviculture,but also an ideal experimental organism for scientific research. Female Artemia produce offspring in both ovoviviparity and viviparity approach. In order to reveal the mechanism of different reproductive modes of Artemia, the reproductive differential transcriptomes of parthenogenetic Artemia were constructed, and bioinformatics analysis were performed to screen reproductive differential expression genes,and the gene expression patterns were studied using qRT-PCR. Transcriptome sequencing showed 1452 differentially expressed genes, including 601 up-regulated genes and 851 down-regulated genes. According to GO function classification,1243, 306 and 530 unigene were annotated into biological process, cell composition and molecular function respectively. KEGG enrichment analysis showed that differential genes were significantly enriched in antigen processing and ribosome pathways. Combined with transcriptome data and qRT-PCR analysiss, six reproductive-related genes were screened and verified. The results showed that all the six reproductive-related genes have higher expression in oviparous Artemia than in ovoviviparous Artemia. The results of this study provide basic information for the study of molecular mechanism regulation of reproductive pattern of parthenogenetic Artemia, and help to improve the reproductive biological theory of Artemia.