The small RNA universe of Capitella teleta

RNAi is an evolutionarily fluid mechanism with dramatically different activities across animal phyla. One major group where there has been little investigation is annelid worms. Here the small RNAs of the polychaete developmental model, Capitella teleta are profiled across development. As is seen with nearly all animals, several hundred microRNAs were found with ~250 novel species. Greater miRNA diversity was associated with later stages consistent with differentiation of tissues. Outside miRNA, a distinct composition of other small RNA pathways was found. Unlike many invertebrates, an endogenous siRNA pathway was not observed, indicating pathway loss relative to basal planarians. No processively generated siRNA-class RNAs could be found arising from dsRNA precursors. This has significant impact on RNAi technology development for this group of animals. Unlike the apparent absence of siRNAs, a significant population of piRNAs were observed. For many piRNAs phasing and ping pong biogenesis pathways were identified. Interestingly, piRNAs were found to be highly expressed during early development, suggesting a role in regulation of the maternal transcriptome. Critically, the configuration of RNAi factors in C. teleta is found in other annelids and mollusks, suggesting that similar biology is likely present in the wider clade. This study is the first providing comprehensive analysis of small RNAs in annelids.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012E41ECE2D24B9B7444E92BEB842337EE31407CC3B
Metadata Access https://data.blue-cloud.org/api/collections/E41ECE2D24B9B7444E92BEB842337EE31407CC3B
Provenance
Instrument NextSeq 500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2022-02-26T00:00:00Z