This data set includes fish biomass data collected during the RV ALKOR cruise AL614 cruise conducted in June 2024 (6th to 15th of June) in the Western Baltic Sea by the University of Hamburg. The cruise was part of the 5-year MARSYS teaching cruise program of the University of Hamburg GFP20-1_047. The cruises are designed to train students in sampling methods targeting the different compartments of a marine ecosystem. Fish biomass was taken after sorting through a complete fish haul into the single species and weighing them on a scale on board.