This dataset is a component of the PAN-Arctic data collection of benthic BIOtas (PANABIO) and has been collated during the Census of Marine Life project Arctic Ocean Diversity (ArcOD; http://www.arcodiv.org/). It contains a total of 1,520 wet biomass records of 150 megabenthic taxa (species to genus level) identified in 70 otter trawl catches taken at 70 stations located in the Chukchi Sea at water depths of 15–64 m during a cruise in 1976. The dataset is also available in a PostgreSQL-based data warehouse that can be accessed and queried through an open-access frontend web service at https://critterbase.awi.de/panabio.