Two Slocum gliders (units 331 and 439) were deployed during RRS Discovery expedition DY081 on July 17th 2017 at 62.9°N, 52.6°W, approximately 40 km off the Greenland shelf break, travelled North along the coast in a zig-zag pattern between the shelf and deep waters, and were recovered 8 days later from 63.7°N, 53.1°W and 62.9° N, 52.7°W respectively on July 24th 2017. Gliders profiled from the surface to 1000 m, except during the two excursions onto the shelf, once south and once north of the Godthåb Trough, where they followed the bathymetry. Each glider was fitted with a pumped CTD and bio-optical sensors (WET Labs puck). These bio-optical sensors measure optical backscattering (in the form of volume scattering function), chlorophyll fluorescence, and UV fluorescence for fluorescing dissolved organic matter (FDOM), a subset of coloured organic matter (CDOM). This dataset contains raw and processed/gridded data files from the glider deployments. The raw data are contained as .dbd and .ebd files in raw_data.zip folders for each of glider unit 331 and 439. The data were processed using the SOCIB glider toolbox (https://github.com/socib/glider_toolbox) and saved as a NetCDF (processed.nc) with the following variables: longitude, latitude, time (Julian Day), pressure, eastward velocity, northward velocity, temperature (not thermally corrected), salinity (not thermally corrected), chlorophyll fluorescence (not corrected for quenching), coloured organic matter (cdom), backscatter (volume scattering function) and oxygen concentration. The expedition report is provided and metadata can be found in the processed/gridded data files.