Normal-to-malignant transformation in human cells, still an ill-understood process, is intimately related to their biomechanical properties, which are strongly dependent on the intracellular water structural and dynamical profiles. Indeed, these are known to play a fundamental role in normal cellular activity, namely through maintenance of the three-dimensional architecture and functional conformation of biopolymers. Building on the success of previous experiments on the impact of an anticancer drug on the cytomatrix of breast cancer cells [1,2], the present study aims to apply QENS for probing changes in intracellular water dynamics between healthy and cancer human cells. This is an innovative approach for unveiling particular features of malignant cells and attain specific spectroscopic signatures of cancer, aiming at a better understanding of the normal-to-cancer transition.