The spatial variation of the persistence length along Widom-601 DNA

DOI

The persistence length of DNA is a quantity used to define the flexibility of the molecule. It is a key parameter to describing the conformation of DNA and, as a result, many biological functions of the molecule including how it packs and its interaction with proteins and membranes. The persistence length is probably not constant along the length of the molecule, however. One biological function that may be strongly influenced is histone positioning, which may be driven by local variations in the persistence length. The commonly investigated Widom-601 DNA molecule is known to have a strong histone positioning effect. Spatial variations in the persistence length will result in changes in the conformational structure of DNA, and these will be observable in scattering experiments. We propose to use SANS to measure the conformational structure of Widom-601 in solution as a function of temperature to determine the spatial variation in the persistence length along its helical axis.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.9-13-592
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.9-13-592
Provenance
Creator Rodriguez, Adrian Gonzalez; Wildes, Andrew; Angelov, Dimitar; Orero, Jessica Valle; Van Eijck, Lambert; Porcar, Lionel; Johnson, Mark Robert; Roda, Marta Marty; Peyrard, Michel; Theodorakopoulos, Nikos; Tonchev, Ognyan; Lopez, Santiago Cuesta
Publisher Institut Laue-Langevin
Publication Year 2015
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 2 GB
Version 1
Discipline Particles, Nuclei and Fields