Recently a generic method for producing polymer-supported lipid bilayers (pSLBs) directly from cell-derived native membrane vesicles (NMVs) was discovered. These pSLBs contain essentially all the naturally occurring cell-membrane components of the donor cell line or organelle while still retaining transmembrane protein mobility and activity. These surfaces offer a new paradigm in SLB-based biomimetic surfaces and bioanalytical sensor design. While fluorescence microscopy studies have indicated that there is at minimum a 5 nm hydration layer between the pSLB and the underlying substrate, the use of neutron reflectivity is expected to provide better insight into the architecture of these complex hybrid pSLBs.