Dataset contain two types of measurements: AFM measurements taken with an AFM NX20 from Park and resistance measurements taken with 2-Point Prove and 4-Point Prove methods. The data is organized in the following folders (indicated as “[FOLDERNAME]”):

“AFM”: Includes the AFM measurements taken for the thickness characterization. The folder includes a Resume.xlsx file that resumes the thickness of the sensors UTMFs. Inside this folder there is a subfolder “Samples 2025” with the Raw AFM data.

Inside “Samples 2025” there are subfolders for each sensor thickness “2nm”, “3nm”, “4nm”, and “8nm”. There is also a folder called “40nm” which includes the AFM measurements on thick gold ribbons to characterize the SH-PEG-COOH self-assembled monolayer (SAM) formation, before and after SAM formation. In a similar way, the folder “Thickfilm” includes the measurements on thick gold ribbons to characterize the SH-PEG-Biotin SAM with 10kDa and 400Da molecular weight, before and after the SAM formation. All AFM data is saved in .tiff files (standard format generated by the Park software) as well as .txt files generated during the data analysis using Gwyddion, which includes the height profiles and averaged heights.

Folders “Air”, “COOH_Thiol_PEG”, “MilliQ”, and “Strep-Biotin_Thiol_PEG” have the resistance measurements taken with the 2-Point Prove method and using the created LabVIEW software, in the fabricated chemiresistors.

The folders “Air”, “COOH_Thiol_PEG”, and “MilliQ” have the same subfolder structure. First subfolders containing the thickness of the sensor UTMF: “2nm”, “3nm”, “4nm”, and “8nm”.

Inside each “Xnm” folder, subfolders indicating when the sample(s) (chemiresistor(s)) was/were fabricated, and inside these folders, subfolders indicating when the measurement was done.

Inside “Strep-Biotin_Thiol_PEG” there are two subfolders indicating the SAM formed “Biotin_Thiol_PEG-10K” and “Biotin_Thiol_PEG-400”.

Inside each “Biotin_Thiol_PEG-X” folder, there are two subfolders, “SAM” and “Strep.Con”, with subfolders indicating when the sample(s) (chemiresistor(s)) was/were fabricated, and inside these folders, subfolders indicating when the measurement was done.

“SAM” folder have the data of the “Biotin_Thiol_PEG-X” SAM formation, and “Strep.Con” folder have the data of each Streptavidin concentration measured.

For each 2-Point Prove measurement, three files are created. For all the files in the data set, the name structure is the same: “[Time of the measurement end][Data showed][Day when the measurement end]_[Custom name].txt”. The custom name includes info of the time of sample, fabrication data and analyte measured. Only one type of data was used in the paper; “Rvstime”. This .txt files contain tabular data in two columns indicating the measured resistance and the relative time of the measurement.

Lastly, a file called “Rs.xlsx” contain the sheet resistance measurements taken with a 4-Point Prove on UTMFs with different thicknesses.

Origin, 2017

Gwyddion, 2.67

Needed software: For data adquisition, it was used a LabVIEW code done by one of the authors. The for the AFM measurements a software given by the manufacturer (Park) For the data analysis and figures I used Origin 2017 and Gwyddion, but other software can be used.