<p>The inner structure of polymeric microgels critically influences their responsiveness and potential applications, yet remaining challenging to resolve at molecular resolution. In this work, a structural characterization of thermoresponsive copolymer microgels is provided by integrating small-angle neutron scattering (SANS), dynamic light scattering (DLS), and nuclear magnetic resonance (NMR) measurements with multi-scale simulations. Specifically, Poly(N-isopropylacrylamide-<em>co</em>-N-isopropylmethacrylamide), P(NIPAM-<em>co</em>-NIPMAM), copolymer microgels, in which a random monomer distribution is conventionally assumed, are considered. By synthesizing different samples, including isotopically labeled microgels via selective deuteration, the microgels swelling behavior is probed and distinct polymer-specific signatures are revealed. To elucidate their internal distribution, monomer-resolved microgel simulations are performed across different copolymer models. A direct comparison between experimental and numerical form factors provides evidence of preferential organization into block structures, challenging the prevailing view of random distribution. <sup>13</sup>C-NMR experiments confirm NIPAM-rich blocks and atomistic simulations link this unexpected block-like architecture to distinct local hydrogen-bonding patterns. This integrated approach provides the first direct evidence of preferential block formation in P(NIPAM-<em>co</em>-NIPMAM) microgels. Beyond this system, these results establish a generalizable strategy for unveiling hidden structural order in copolymer microgels, offering new strategies to tailor their design and to enhance control of material responsivity.</p>
