Microbe abundance and biomass in waters of the Cretan Sea at Station MARCH-1995-D7


10-30 ml subsamples were concentrated on 25 mm black polycarbonate filters (0.6 µm for NF, 0.2 µm for B), stained with DAPI for 10 min and filtered. NF were counted using UV and blue excitation and enumerated. Heterotrophic B were counted using UV excitation. NF were classified in size categories and biovolume was calculated. NF abundance data were converted into C biomass using 220 fgC µm-3. Bact abund data were converted into C biomass using 20 fgC cell-1. BP C was estimated by the 3H-leucine approach according to Kirchman et al. (1986) and Kirchman D.L., 1993. Leucine incorporation as a measure of biomass production by heterotrophic bacteria. In: Kemp P.F. et al. (eds), 1993. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Ann Arbor, 509-512). At each depth duplicate samples and a control one were incubated with 1 nM L-[4,5 3H]-leucine (specific activity 128 Ci/mmol) and 18 nM non-radioactive leucine. Samples were incubated in the dark at in situ temperature.

DOI https://doi.org/10.1594/PANGAEA.693707
Related Identifier https://doi.org/10.1594/PANGAEA.728763
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.693707
Creator Psarra, Stella; Tselepides, Anastasios
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Hellenic Center of Marine Research, Institut of Oceanography, Greece
Publication Year 2008
Funding Reference European Commission, MAS20092
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 21 data points
Discipline Earth System Research
Spatial Coverage (25.100 LON, 35.750 LAT)