Eight consistently amplifying markers were used for Cetraria aculeata. Samples of Usnea aurantiacoatra and U. antarctica were genotyped using 21 and 22 microsatellites markers, respectively. Detailed information on primers and PCR amplification can be found in Lutsak et al. (2016) and Lagostina et al. (2017). PCR amplicons were electrophoresed using an Applied Biosystems 3730 sequencer, with the LIZ 500 (C. aculeata)or LIZ 600 (Usnea sp.) size standards (Applied Biosystems, Waltham, Mass., USA). Allele sizes were manually scored using the Geneious 10 microsatellites tool (Kearse et al. 2012). Those SSR markers were used to study the effects of dispersal strategy and phylogeographic history on the population genetic structure of Antarctic lichens.

Supplement to: Lagostina, Elisa; Dal Grande, Francesco; Scur, M; Lorenz, Andrea; Andreev, M; Ruprecht, U; Søchting, U; Sancho, L G; Wirtz, Nora; Rozzi, R; Printzen, Christian (in prep.): Genetic structure and gene flow of three lichens forming fungi in the Maritime Antarctic and southern South America. Journal of Biogeography