Sequencing of 12 pooled samples of Arctocephalus gazella (Antarctic fur seal) transcriptome, using 454-Titanium

RNA from skin samples of 12 Arctocephalus gazella (Antarctic fur seal) individuals was pooled into one sample, then normalised cDNA was synthesised using the Evrogen SMART cDNA synthesis service. The cDNA was sequenced on the Roche 454 titanium platform. The resulting reads were assembled and BLAST mapped to the dog genome and the non-redundant protein dataset. Finally, putative microsatellites and SNPs were identified.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012582362E39931D0C263940518F5CEF586105F2127
Metadata Access https://data.blue-cloud.org/api/collections/582362E39931D0C263940518F5CEF586105F2127
Provenance
Instrument 454 GS FLX Titanium; LS454
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2025
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2011-01-21T00:00:00Z