-
Pyrococcus furiosus primed spacer uptake
In CRISPR-Cas, DNA fragments are captured and used as immunological memories. This uptake process can be primed by recognition of an existing target. To study the mechanism in... -
Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping
N4-acetylcytidine (ac4C) is an ancient and highly conserved RNA modification, present on tRNA, rRNA and recently investigated in eukaryotic mRNA. We report ac4C-seq, a chemical... -
Pyrococcus furiosus strain:JFW02 Targeted Locus (Loci)
Amplicon sequencing of Pyrococcus furiosus CRISPR arrays -
Pyrococcus furiosus Cas mutants, targeted sequencing of CRISPR loci
Targeted sequencing of CRISPR loci in Pyrococcus furiosus revealed patterns of spacer acquisition. These patterns differ among Cas mutants. -
CopR is a global regulator of copper homeostasis in Pyrococcus furiosus
In several archaea, the Cu-transporting ATPase and the copper chaperone are arranged in a conserved copper resistance gene cluster (cop), which also contains an additional gene,... -
Endogenous RNA bind-n-seq (endo-bind-n-seq): determining RNA interaction moti...
RNA endo-bind-n-seq allows for the identification of RNA elements that are bound by RBPs, which are isolated from their physiological environments using immunoprecipitation and...
