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MBD2 modulates heterochromatin architecture and composition
Heterochromatin (HC) is essential for nuclear integrity, genome stability, and gene regulation with pericentric heterochromatin (PCH) playing a key role. However, the mechanisms... -
Histone variant macroH2A1 regulates synchronous firing of replication origins...
MacroH2A has been linked to transcriptional silencing, cell identity, and is a hallmark of the inactive X chromosome (Xi). However, it remains unclear whether macroH2A plays a... -
Uptake of substances into living mammalian cells by microwave induced perturb...
Delivering foreign molecules and genetic material into cells is a crucial process in life sciences and biotechnology, resulting in great interest in effective cell transfection... -
DNA choreography: correlating mobility and organization of DNA across differe...
The dynamics of DNA in the cell nucleus plays a role in cellular processes and fates but the interplay of DNA mobility with the hierarchical levels of DNA organization is... -
Developmental changes in genome replication progression in pluripotent versus...
DNA replication is a fundamental process ensuring the complete and accurate maintenance of the genome each time cells divide. This is particularly relevant early in development... -
The human Timeless-Tipin complex assures the replication fork protection ahea...
The accuracy of replication is one of the most important mechanisms ensuring stability of the genome. The fork protection complex prevents premature replisome stalling and/or... -
Developmental changes in genome replication progression in pluripotent versus...
DNA replication is a fundamental process ensuring the complete and accurate maintenance of the genome each time cells divide. This is particularly relevant early in development... -
Replisome loading reduces chromatin motion independent of DNA synthesis
Chromatin has been shown to undergo diffusional motion, and during active gene transcription the RNA polymerase activity negatively affects chromatin motion. However, the... -
Cytosine base modifications regulate DNA duplex stability and metabolism
DNA base modifications diversify the genome and are essential players in development. Yet, their influence on DNA physical properties and the ensuing effects on genome... -
Single cell data
Time series data as well as time of S-phase entry and cell division for individual cells under the different experimental conditions used in the published study -
The chromatin architectural protein CTCF is critical for cell survival upon i...
CTCF is a nuclear protein initially discovered for its role in enhancer-promoter insulation. It has been shown to play a role in genome architecture and in fact its DNA binding... -
Analysis code
R code to analyze single cell, Luminex and immunofluorescence data -
MeCP2 heterochromatin organization is modulated by arginine methylation and s...
Rett syndrome is a human intellectual disability disorder that is associated with mutations in the X-linked MECP2 gene. The epigenetic reader MeCP2 binds to methylated cytosines... -
Single cell trajectories
This dataset has no description
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MeCP2-induced heterochromatin organization is driven by oligomerization-based...
Heterochromatin is the highly compacted form of chromatin with various condensation levels hallmarked by high DNA methylation. MeCP2 is mostly known as a DNA methylation reader... -
Developmental differences in genome replication program and origin activation
To ensure error-free duplication of all (epi)genetic information once per cell cycle, DNA replication follows a cell type and developmental stage specific spatio-temporal... -
Single cell data
Time-resolved analysis of nuclear-to-cytoplasmic SMAD2 ratio in individual cells. For some datasets, data regarding motility and cell death is included as well. Data is provided... -
Isoform-specific and ubiquitination dependent recruitment of Tet1 to replicat...
Oxidation of the epigenetic DNA mark 5-methylcytosine by Tet dioxygenases is an established route to diversify the epigenetic information, modulate gene expression and overall...
